Professor Rune Blomhoff
Avdeling for ernæringsvitenskap, Institutt for medisinske basalfag, Det medisinske fakultet, Universitetet i Oslo
E-mail: rune.blomhoff@medisin.uio.no

 

 

Oslo Antioxidant Study
 Dietary antioxidant intervention in smoking middle-aged and elderly men

Participating scientist
  • Prof. Rune Blomhoff, prof. Christian A. Drevon, prof. Asim Dutta-Roy, Prof. Andrew Collins, prof. Jan Øivind Moskaug, Anette Karlsen, Mari Myhrstad, Siv Kjølsrud Bøhn, Amrit Kaur Sakhi, Asgeir Brevik at the Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo
  • Prof. Serena Tonstad, Mette Svendsen at the Department of Preventive Cardiology, Ullevål University Hospital and Mary-Ann Sommernes
  • Ingebjørg Seljeflot and Harald Arnesen at the Center for Clinical Research and Department of Cardiology, Ullevål University Hospital
  • Prof. Petter Laake, prof Magne Thoresen, Department of Medical Statistics, Institute of Basic Medical Sciences, University of Oslo
  • Prof. Karin Haffner, Siv Fagertun Remberg, Grethe Iren Borge, The Agricultural University of Norway
  • Other scientsits: ars Ove Dragsted, Marit Holden, Siv Haugen Tunheim, Iris Erlund, Mauro Serafini

Aim of the study

The aim of the study is to test whether a diet enriched in "total antioxidants" or kiwi fruits support the antioxidant defense, reduce oxidative damage, improve biomarkers for oxidative stress or improve other disease related markers (endothelial function, platelet function) in a population with oxidative stress

Study population
  • Smoking men age 50 years or more
  • Recruited at the Ullevål Hospital.
  • Total 102 individuals

Study design

  • 3 groups

  • 4 weeks run-in and 8 weeks intervention

  • Blood samples before and after intervention

Intervention
  • Antioxidant group: High intake of a variety of antioxidant-rich plant products (walnuts, pomegranates, blueberries, blackberries, ginger, fruit and berry juices, green tea, kiwi fruits, tomatoes, spices, blue potatoes, blue broccoli, red cabbage etc).
  • Kiwi group: 3 kiwi fruits per day
  • Control group: Individuals are free to eat whatever they prefer, but they should restrict intake of antioxidant-rich foods.

 
  Totalt antioksidant-innhold
Grønn te, 7 kopper 25,6
Mana juice, Nype & Appelsin, 5 bokser a 330 ml 42,4
Mana juice, Blåbær & Aronia , 5 bokser a 330 ml 33,0
Mana juice, Tranebær, bringebær & drue , 5 bokser a 330 ml 11,1
Valnøtter, 200 g 41,9
Solsikkefrø, 100 g 6,4
Granatepler, 200 g 22,7
Blåbær, 200 g 16,5
Bjørnebær, 200 g 10,1
Jordbær, 200 g 4,3
Bringebær, 200 g 3,1
Rosenkål, 200 g 2,3
Brokkoli, 200 g 1,2
Rødkål, 200 g 3,8
Grønnkål, 200 g 5,3
Poteter, blå Congo, 150 g 1,2
Tomater, 700 g 2,2
Nypesyppe, 200 ml 5,6
Mørk sjokolade, 100 g 13,4
Pecanøtter, 100 g 9,0
Blå druer, 200 g 4,8
Rød chilli, 10 g 0,3
Rosmarin krrydder 1,8
Timian krydder 1,8
Oregano krydder 1,2
Blåbær syktetør, usukret, 345 g 9,1
Blåbærsaft, usukkret, 660 ml ufortynnet 43,6
Totalt mengde antioksidanter iløpet av uken 323,6

End point including:
  • Antioxidant and oxidative stress profile
  • Redox status
  • Expression of stress and ageing related genes in WBC by DNA macroarrays
  • DNA microarray analysis on pooled WBC samples
  • Endothelial function/inflammation
  • DNA damage and repair
  • Platelet function
  • Plasma lipids
  • Blood pressure

Acknowledgements
This project is supported by grants from the Norwegian Research Council, The Norwegian Cancer Society, Throne Holst Foundation, Fellesjuice and others. We are grateful to Fellesjuice for developing and supporting free of charge MANA juice used in the study.